摘要
目的:考察广西不同采收期喜树果中喜树碱成分的动态积累情况,确定喜树果药材最佳采收期。方法:采用高效液相色谱法(HPLC)测定广西部分地区同一植株不同采收期喜树果中喜树碱含量。色谱条件:色谱柱:Dikma Diamonsil(钻石)C18柱(4.6mm×250mm,5μm),流动相:甲醇—水(55∶45),流速:0.8mL/min,柱温:室温,检测波长:254nm。结果:测得喜树碱在0.112～0.672μg范围内有良好线性关系,平均回收率为102.16%,RSD=2.91%(n=6)。不同采收期喜树果中喜树碱含量在0.065%～0.21%之间。结论:广西不同采收期喜树果中喜树碱成分的动态积累有一定差异,广西产喜树果最佳采收期应为每年的8,9月份。
Objective：To inspect the dynamic accumulation of camptothecin in common camptotheca fruit from different areas and different growing period in Guangxi.At last,to establish the best harvest period of common camptotheca fruit.Methods：The HPLC method was used,chromatography conditions were C18 column（4.6 mm×250 mm,5 μm）,the CH3OH-H2O（55∶45） as a mobile phase,the flowrate was 0.8 mL/min,the column temperature was room temperature and detection wavelength at 254 nm.Results：There was a good linear relationship for camptothecin within the range of 0.112~0.672 μg.The average recovery was 102.16%,RSD=2.91%（n=6）.Its content is between 0.065%~0.21%.Conclusion：The dynamic accumulation of camptothecin in Fructus Camptothecae has certain differences from different growing period in Guangxi,August or September is the best harvest times.
出处
《广西医科大学学报》
CAS
2011年第5期678-680,共3页
Journal of Guangxi Medical University
基金
广西自然科学基金资助项目(No.桂科自0542055)
关键词
喜树果
喜树碱
动态积累
高效液相色谱法
common camptotheca fruit
comptothecin
dynamic accumulation
HPLC
