摘要
目的:建立黔产喜树叶的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Gemini-NX C_(18),流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为1.0 m L/min,检测波长为370 nm,柱温为30℃,进样量为10μL。以山柰素为参照物,测定14批喜树叶药材的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A)进行共有峰指认、相似度评价,并进行聚类分析。结果:14批喜树叶药材的HPLC图谱共有10个共有峰,13批喜树叶药材与对照图谱的相似度>0.90,1批与对照图谱相似度<0.90。喜树叶药材样品可归为3大类。结论:该研究所建指纹图谱可为黔产喜树叶的鉴别和质量评价提供参考。
OBJECTIVE： To establish HPLC fingerprint for the leaves of Camptotheca acuminante in Guizhou. METHODS： HPLC method was performed. The determination was performed on Gemini-NX C18 column with mobile phase consisted of acetoni- trile-0.2% phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 370 nm, and the column temperature maintained at 30 ℃. The sample size was 10 μL. Using sorbitol as a reference, HPLC fingerprints of 14 batch- es of the leaves of C. acuminante were determined. The chromatographic fingerprint was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A） in terms of common peak indentification, similarity evaluation and cluster anal- ysis. RESULTS ： There were 10 common peaks in HPLC fingerprints for 14 batches of the leaves of C. acuminate. And the similari- ty of 13 batches of the leaves of C. acuminate was greater than 0.90, and that of another one was less than 0.90. The leaves of C. acuminate were classified into 3 groups. CONCLUSIONS： The established fingerprint can provide reference for identification and quality evaluation of the leaves of C. acuminate.
出处
《中国药房》
CAS
北大核心
2017年第24期3412-3414,共3页
China Pharmacy
基金
贵州省中药材
民族药材质量标准研究课题(No.DBXZYC52032)
关键词
喜树叶
指纹图谱
聚类分析
高效液相色谱法
The leaves of Camptotheca acuminante
Fingerprint
Cluster analysis
HPLC
