摘要
对酒精高产酿酒酵母GGFS16和GJ2008单倍体原生质体的形成与再生条件进行了研究。结果表明,在菌龄8h、0.2%β-巯基乙醇和0.1%EDTA-Na2预处理20min、1.5%的蜗牛酶37℃处理30min以及使用浓度170g/L的蔗糖作为渗透压稳定剂的条件下,GGFS16和GJ2008单倍体原生质体的形成率和再生率均达到最大。形成率分别是98.14%和97.19%,再生率分别是17.29%和15.78%。
The conditions of formation and regeneration of protoplast from two high-alcohol-yield Saccharomyces cerevisiae haploid strains GGFS l 6 and GJ2008 were optimized. The optimum conditions were as follows： the haploid cultured for 8h, hydrolyzed with 1.5% snailase at 37℃ for 30 rain after pretreatment by 0.2% mercaptoethanol and 0.1% EDTA-Na2; 170g/L sucrose were used as osmotic stabilizer. Under these conditions, the formation rate of protoplast from the GGFS 16 and G J2008 haploid strains reached 98.14% and 97.19%, respectively. The regeneration rate reached 17.29% and 15.78%, respectively..
出处
《中国酿造》
CAS
北大核心
2011年第11期42-46,共5页
China Brewing
基金
广西科技厅项目(桂科攻0782003-2)资助
广西青年科学基金项目(桂科青0991010)资助
关键词
酿酒酵母
酒精高产
原生质体
形成率:再生率
Saccharomyces cerevisiae
high-alcohol-yield
protoplast
formation rate
regeneration rate
