摘要
实验研究了二倍体酿酒酵母(Saccharomyces cerevisiae)菌株GJ2008和GGFS16的孢子形成和分离条件,并获得了相应的单倍体细胞。实验结果表明,酿酒酵母GJ2008和GGFS16在McClary培养基上22℃培养7d,产孢率分别为91.46%和87.20%。在此条件下,以浓度为0.3g/L的蜗牛酶37℃水浴处理,孢子释放率分别为66.15%和67.35%。对获得的单倍体菌株进行发酵实验,并测定其酒精发酵性能,为融合实验做好铺垫。
In order to obtain the haploids of Saccharomyces cerevisiae for the protoplast fusion, the conditions of sporulation and isolation of haploids of S. cerevisiae strains GGFS 16 and G J2008 were studied. The results showed that the optimum sporulation medium and incubation time of GGFS 16 and G J2008 were on McCLary medium and 7d, respectively. The optimum cultivation temperature was 22℃. Under these conditions, spore production rate of GGFS16 and G J2008 were 91.46% and 87.20%, respectively. Further study showed that spore production rate of GGFSI6 and G J2008 were 66.15% and 67.35%, respectively, when the concentration of snailase was 0.3g/L and enzymatic treatment time was 8h. Moreover, ethanol fermentation experiments of diploids of S. cerevisiae GGFS16 and GJ2008 were conducted and their fermentation performances were measured.
出处
《中国酿造》
CAS
北大核心
2011年第9期80-84,共5页
China Brewing
基金
广西科技厅项目(桂科攻0782003-2)
关键词
酿酒酵母
子囊孢子
单倍体
产孢条件
单倍体分离
Saccharomyces cerevisiae
ascospore
haploid
spore production conditions
haploid isolation
