摘要
利用具有高分辩率和高灵敏度的毛细管电泳 -电化学检测方法测定乳糖、麦芽糖及其水解产物的浓度随反应时间变化的规律。在H2SO4 催化下,对不同温度时乳糖、麦芽糖水解反应速率进行了测定,在85℃、90℃和95℃时乳糖的水解反应速率常数分别为13.3×10-3、30.1×10-3 和52.5×10-3 min-1;而麦芽糖在85℃、95℃和100℃时的反应速率常数分别为17.7×10-3、84.6×10-3 和150×10-3 min-1;并据此分别求得反应活化能Ea 为161.1kJ/mol、194.7kJ/mol。
The concentration of lactose and maltose and their hydrolysis products were monitored by ca_pillary electrophoresis-electrochemical detection due to its high resolution and high sensitivity of detection. The reaction rate constant of lactose and maltose hydrolysis at different temperature in 0.75 mol/L H2SO4 were obtained from the concentration change of lactose and maltose during the process of hydrolysis. At 85 ℃, 90 ℃ and 95 ℃, the measured rate constants of lactose hydrolysis were 13.3×10-3 min-1, 30.1×10-3 min-1 and 52.5×10-3 min-1, respectively, from which the activation energy of the reaction(Ea) value of 161.1 kJ/mol was derived. At 85 ℃, 95 ℃ and 100 ℃, the measured rate constants of maltose hydrolysis were 17.7×10-3 min-1, 84.6 ×10-3 min-1 and 150×10-3 min-1, respectively, Ea value obtained was 194.7 kJ/mol.
出处
《分析测试学报》
CAS
CSCD
北大核心
2001年第1期16-19,共4页
Journal of Instrumental Analysis
基金
中国科学院长春应用化学研究所电分析化学开放实验室资助项目
关键词
毛细管电泳
电化学检测
乳糖
麦芽糖
反应速率常数
水解
化学动力学
Capillary electrophoresis
Electrochemical detection
Lactose
Maltose
Reaction rate constant
Hydrolysis reaction
