摘要
目的:建立HPLC法同时测定注射用冠心宁中原儿茶醛、阿魏酸、丹酚酸B含量的方法。方法:色谱柱:Kromasil C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.6%磷酸溶液梯度洗脱,检测波长:286 nm。结果:原儿茶醛在0.022～0.540μg(r=0.999 3)范围内与峰面积呈良好的线性关系,平均回收率为99.10%,RSD=1.45%;阿魏酸在0.022～0.550μg(r=0.999 7)范围内与峰面积呈良好的线性关系,平均回收率为99.89%,RSD=1.01%;丹酚酸B在0.206～2.506μg(r=1.000 0)范围内与峰面积呈良好线性关系,平均回收率为101.45%,RSD=1.32%。结论:该方法准确、重复性好,可用于注射用冠心宁的质量控制。
Objective： To establish an HPLC method for the simultaneous determination of protocatechuie aldehyde, ferulic acid ancl salvianolie acid B in Guanxinning powder injections. Method： The determination was carried out on Kromasil C18 column （250 mm × 4. 6 ram,5μm）. Gradient elution method was used with CH3CN-0. 6% H3PO4 as the mobile phase at a flow rate of 1.0 ml·min^-1. The UV detection wavelength was 286 nm. Re.suit： The linear range of protocatechuic aldehyde, ferulie acid and salvianolic acid B was 0. 022-0. 540 μg, r = 0. 999 3 ）, 0. 022-0. 550μg （ r = 0. 999 7 ） and 0. 206-2. 506 μg （ r = 1. 000 0 ） with the average recovery of 99. 10% （RSD = 1.45% ） ,99. 89% （RSD = 1.01% ） and 101.45（RSD = 1.32% ） ,respectively. Conclusion： The method is accurate and repeatable which provides a method for the quality control of Guanxinning powder injections.
出处
《中国药师》
CAS
2011年第10期1469-1471,共3页
China Pharmacist
关键词
注射用冠心宁
原儿茶醛
阿魏酸
丹酚酸B
含量测定
高效液相色谱法
Guanxinning powder injections
Protoeatechuie aldehyde
Ferulic acid
Salvianolie acid B
Determination
HPLC
