摘要
目的:研究大孔吸附树脂法纯化愈肾颗粒总黄酮最佳工艺。方法:以总黄酮的比吸附量、解析率、洗脱率等为指标,选用7种大孔吸附树脂对愈肾颗粒总黄酮进行纯化。结果:HPD100型大孔吸附树脂对愈肾颗粒的总黄酮具有较好的分离纯化能力。最佳工艺条件为2 BV的愈肾颗粒供试品溶液为上样量(含生药量为1 g.mL-1),吸附速率为1 BV.h-1,分别用水2BV、30%乙醇3 BV、50%乙醇4 BV洗脱,洗脱流速4 BV.h-1,合并30%乙醇和50%乙醇洗脱液,浓缩,即为纯化的总黄酮。结论:纯化后总黄酮含量提高到76%以上,HPD100型大孔吸附树脂可较好的纯化愈肾颗粒总黄酮。
Objective： To study on optimum purification technology of total flavonoids from Yushen granules by macroporous resin.Method： With adsorption capacity,desorption rate and elution rate as indexes,purified total flavonoids of Yushen granules by seven macroporous resin.Result： HPD100 macroporous resin showed good separation and purification capabilities of total flavonoids from Yushen granules.The optimum technology conditions were as follows：2 BV sample（dose of raw drug was 1 g·mL-1） of Yushen granules formula was passed through the column with flow rate of 1 BV·h-1,eluted by 2 BV water,3 BV 30% ethanol,4 BV 50% ethanol respectively,elution flow rate was 2 BV·h-1.Combined 30% and 50% ethanol elutes were concentrated to yield the purification of total flavonoids.Conclusion： The purity of total flavonoids was up to 76%.HPD100 could be well used in purifying total flavonoids from Yushen granule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第20期8-10,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
十一五重大新药创制项目(2009ZX09103-394)
关键词
大孔吸附树脂
总黄酮
愈肾颗粒
纯化
macroporous resin
total flavonoids
Yushen granules
purification
