摘要
目的应用大孔吸附树脂纯化人参提取物中人参皂苷,并对其工艺进行优化。方法以人参总皂苷的含量为指标,比较不同型号树脂和不同工艺条件对人参皂苷的分离纯化能力。结果 X-5树脂具有较好的吸附和解吸附性能,其最佳工艺条件为上样液质量浓度为5.99mg/ml,上样体积为2倍柱体积,吸附流速为1.1ml/min,用70%乙醇洗脱,用量5倍柱体积,洗脱流速为1.0ml/min,人参总皂苷纯度可达60%以上。结论该工艺简便,适宜于人参总皂苷的工业化分离纯化。
Objective To optimize the refining technology for total saponin of panax ginseng by macroporous resin. Methods Based on the content of total saponin of panax ginseng, comparison of different types of resin and different technological conditions on the separation and purification capacity of total saponin of panax ginseng. Results X-5 macroporous resin＇s adsorption and disadsorption ability was better, the best technological conditions were 70% ethanol as elution agent, volume of elution agent was 5 BV and the sample volume agent was 2 BV, concentration of sample was 5.99 mg/ml and the flow velocity of adsorption was 1.1 mE/rain and the flow eluting velocity was 1.0 ml/min. In these condition, purity of total saponin of panax ginseng could reach above 60%. Conclusion Macroporous resin method was easy to separate and purify total saponin of panax ginseng in industry.
出处
《环球中医药》
CAS
2013年第2期84-88,共5页
Global Traditional Chinese Medicine
基金
广东省产学研项目(2011B090400545)
广州市白云区科技支撑计划课题(2009-SZ-44)
关键词
人参总皂苷
大孔树脂
分离纯化
Total saponin of panax ginseng（TSPG）
Macroporous resin
Separation and purification
