摘要
目的 :筛选分离葛根总黄酮的最佳树脂 ,并对影响分离的各种因素进行系统的研究 ,使分离工艺达到最优化。方法 :采用静态与动态的吸附 解吸两种方法 ,用紫外分光光度法测定葛根总黄酮的含量对工艺进行评价。结果 :SP70分离效果最好 ,其最佳工艺为药液浓度 0 .5 g·mL-1 (相当于原生药 )、pH为 5～ 6、上样量为 6 0BV(树脂床体积 ) ,以 2BV/h吸附速率进行吸附 ,4BV的 70 %乙醇、2BV/h的流速进行洗脱效果最佳。经SP70处理后的葛根总黄酮可达 80 %以上。结论 :该法简单可行 ,分离效果好 ,能满足于大生产的要求。
AIM: To explore the optimal separation of the total flavonoid from Radix Puerariae by selecting appropriate macroporous resins and to study systematically the factors which affect separation. METHODS: Static and dynamic adsoption desorption methods were adopted, and evaluated for separating efficiency by measuring the concentration of total flavonoid in Radix Pueraria with UV spectrophotometer. RESULTS: The SP70 macroporous resin had the best separating efficiency when the flavonoid content in the liquid was 0.5g per 1mL equivalemt to raw material. pH5～6. the volume of drug 60BV (resin bed volume) with the adsorption power 2BV/h. and the volume of 70%(mL·mL -1 ) ethanol as eluant 4BV with desorption power 2BV/h. In result. total flavonoid's purity could reach 80%. CONCLUSION: This method is simple and feasible with good effect of separation, which can meet industrial requirements.
出处
《中成药》
CAS
CSCD
北大核心
2004年第10期784-788,共5页
Chinese Traditional Patent Medicine
关键词
大孔树脂
葛根总黄酮
工艺优化
macroporous resin
total flavonoid of Radix Puerariae
optimization of technics
