摘要
目的:用分子生物学方法,对古尼虫草和亚香棒虫草进行研究,对其寄主昆虫CO(Icytochrome oxidase subunit I,细胞色素氧化酶亚基I)和真菌ITS(Internal Transcribed Sequence,内转录间隔区)区的基因序列进行比较,以确定两者亲缘关系。方法:在古尼虫草和亚香棒虫草性状研究的基础上,对两者来源真菌ITS区和寄主昆虫COI基因进行了PCR扩增和序列测定,对序列进行比对分析,并与GenBank核酸序列数据库中的序列进行BLAST检索比对。结果:发现古尼虫草和亚香棒虫草的来源真菌ITS区和寄主昆虫COI基因序列均有较高相似度。结论:古尼虫草和亚香棒虫草有较近的亲缘关系。
Objective： To determine the genetic relationship between Cordyceps gunnii and Cordyceps hawkesii, we conducted a comparative study of the rDNA ITS （Internal Transcribed Sequence ）regions of sources fungi and COl （cytochrome oxidase subunit I, cell pigment oxidation enzyme subunits I ） gene of hostinsects. Methods： On the basis of properties research of Cordyceps gunnii and Cordyceps hawkesii., The rDNA ITS （Internal Transcribed Sequence） regions of sources fungi and COl （cytochrome oxidase subunit I, cell pigment oxidation enzyme subunits I ） gene of hostinsects were amplified and sequenced.The sequences were analyzed and compared with sequences in Genbank database. Results： For sequences of rDNA ITS regions of sources fungi and CO1 gene of hostinsects ,there are high similarities between Cordyceps gunnii and Cordyceps hawkesii. Conclusions： Close relationship exists in Cordyceps gunnii and Cordyceps hawkesii.
出处
《现代生物医学进展》
CAS
2011年第17期3355-3358,3369,共5页
Progress in Modern Biomedicine
关键词
古尼虫草
亚香棒虫草
亲缘关系
ITS
COI
Cordyceps gunnii
Cordyceps hawkesii
Phylogenetic relationship
ITS
COI
