摘要
目的：建立裸鼠脑内人胶质瘤模型。方法：应用自制的脑内注射细胞装置，将人的Ｕ－２５１ＭＧ细胞接种于９只裸鼠脑中。分别于接种后１０，２０，３０，４０和７７ ｄ 处死动物并行病理解剖、ＧＦＡＰ免疫组化及电镜检查。结果：我们建立的模型，肿瘤在脑内生长稳定，其特性同于人脑胶质瘤。结论：接种后２０ ｄ
Objective: To set up human glioblastoma multiform model in nude mice. Methods: The U 251MG cell line was injected into 9 nude mice brains with self prepared intracerebral injecting apparatus. These animals were killed 10, 20, 30, 40 and 77 d after injection, and pathologic analysis were made by light microscope, electron microscope and immunohistologic stain for GFAP. Results: The tumors growing stably in these nude mice brain possessed similar characteristic to human′s one. Conclusion:Twenty days after injection is the appropriate time to use the model.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第10期789-791,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
