摘要
目的:探讨125I治疗人脑胶质瘤的可行性及其机制。方法:体外培养人胶质瘤细胞(SHG-44),采用MTT法检测125I粒子对SHG-44细胞增殖抑制的影响;采用立体定向的方法建立大鼠脑内人胶质瘤模型,接种1周后经MRI检测脑内形成实体瘤。将建模成功大鼠随机分成实验组(n=30)与对照组(n=10)。实验组大鼠在当天肿瘤区域植入125I粒子1粒,植入粒子1周后复查MRI,2周后处死大鼠,取对照组、实验组肿瘤周边组织及肿瘤组织做增殖细胞核抗原(PCNA)免疫组化检测。结果:MTT法检测SHG-44细胞经125I粒子照射后,增殖受到明显抑制,接种1周后脑内形成实体瘤;125I可抑制肿瘤细胞生长,延长荷瘤鼠生存期,PCNA基因表达受抑制。结论:125I抑制体外培养的SHG-44细胞生长和颅内接种的脑胶质瘤生长的作用机制可能与抑制PCNA基因表达有关。
Objective To investigate the possibility and mechanism of ^125Ⅰ in treatment of glioma. Methods SHG-44 glioma cells were cultivated in vitro, the inhibitory effect of ~25 1 on SHG-44 cell proliferation was determined by MTT method. The stereotactic method was used to establish the rat intracranial glioma model. The MRI was performed at 1st week after implantation and ^125Ⅰ was implanted in the glioma area, the MRI was performed to measure the diameter of tumor 2 weeks after implantation. The rats were killed after 2 weeks , PCNA gene experession was determined with immunohistological method both in control and experiment group. Results one week after implantation the glioma grew, the results of MTT method showed the growth of the SHG-44 was inhibited, ^125Ⅰ inhibited the expression of PCNA gene and enlonged the rat survival period. Conclusion ^125Ⅰ can inhibit the growth of glioma , the mechanism may be concerned with its inhibitory effect on PCNA gene expression.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期804-807,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题(200505229)
