摘要
目的 探讨樟柳碱和山莨菪碱对急性肺损伤（ＡＬＩ） 的预防作用及其机制。方法 将５０ 只Ｗｉｓｔａｒ 大鼠随机分成５ 组：生理盐水组，油酸模型组，地塞米松预防组，樟柳碱预防组，山莨菪碱预防组。各预防组在注入油酸前３０ 分钟分别于腹腔内注入地塞米松２ ｍｇ／ｋｇ，樟柳碱２ ｍｇ／ｋｇ，山莨菪碱１０ ｍｇ／ｋｇ。以上各组均在注油酸后４ 小时采血测超氧化物歧化酶（ＳＯＤ） 、脂质过氧化物（ＬＰＯ），观察肺组织病理形态学变化。结果 樟柳碱和山莨菪碱预防组血浆ＬＰＯ含量［（９－０９ ±２－０７）μｍｏＩ／Ｌ和（８－６７±２－１０）μｍｏＩ／Ｌ］ 与油酸模型组血浆ＬＰＯ含量［（１２－１８±１－６７）μｍｏＩ／Ｌ］ 比较均明显降低（ Ｐ均＜ ０－０１）。病理组织学观察示樟柳碱和山莨菪碱预防组肺组织病理改变较油酸模型组减轻。结论 樟柳碱、山莨菪碱能改善油酸致ＡＬＩ的氧自由基及肺的病理形态学变化，对ＡＬＩ有预防作用。
Objective To observe the protective effects of anisodine and anisodamine on acute lung injury induced by oleic acid in rats and their mechanisms.Methods Fifty Wistar rats were randomly divided into 5 groups:sodium chlorde group,oleic acid model group,dexamethasone treated group(intraperitoneally with 2 mg/kg dexamethasone at 30 mimutes before oleic acid injection),anisodine treated group(intraperitoneally with 2 mg/kg anisodine at 30 minutes before oleic acid injection),anisodamine treated group(intraperitoneally with 10 mg/kg anisodamine at 30 minutes before oleic acid injection).The changes in superoxide dismutase(SOD),lipid peroxidation(LPO)contents in blood and pathologic histology were observed.Results Plasma LPO levels in anisodine and anisodamine treated group were markedly decreased[LPO:(9 09±2 07)μmoI/L and (8 67±2 10)μmoI/L vs.(12 18±1 67)μmoI/L,all P <0 01]compared with oleic acid model group.Pulmonary tissue damages were diminished by pretreatment with anisodine and anisodamine.Conclusions It is suggested that anisodine and anisodamine have protective effects on the acute lung injury induced by oleic acid. [
出处
《中国急救医学》
CAS
CSCD
北大核心
1999年第11期663-664,共2页
Chinese Journal of Critical Care Medicine
