摘要
１９９３～１９９５年中国对虾发生暴发性流行病，经对患病对虾作组织病理学研究和电镜观察，发现此病以消化系统病变最为严重，肝胰腺、胃及肠道上皮层发生变性和坏死，上皮细胞破坏，细胞核肿大；结缔组织发生水肿，血淋巴细胞浸润．在电镜下，病变组织的细胞核内充满一种杆状病毒，大小为２６０～３１０ｎｍ×９０～１１０ｎｍ，外被双层囊膜，不形成包涵体．纯化的病毒经负染后在电镜下见到的大多数是病毒核衣壳，大小为２８６ｎｍ×６３ｎｍ．用病虾肝胰腺和肠道组织制备的接种液，经３种不同途径人工感染健康对虾，均能引起发病死亡，死亡率为５６．２％～８４．０％，并能从病虾组织中见到一致的杆状病毒．研究表明，引起中国对虾暴发性流行病的病原是一种杆状病毒，其分类地位有待进一步研究确定．
Outbreaks of an epidemic baculoviral disease occurred in Chinese penaeid shrimps and caused heavy economic losses during 1993￣1995. The main histopathological changes of the diseased shrimps included denaturation and necrosis of epithelial layers of the hepatopancreas and gastrointestinal tracts, edema of the connective tissues, and infiltration of the lymphocytes. Under electron microscope, the nuclei of the affected cells were filled with baculovirus particles without inclusion bodies. The intact virions were 260￣310 nm×90￣110 nm in size with double layer envelope. After solvent extraction, the naked virions were 63 nm×286 nm in size. Inoculation of shrimps with the diseased shrimp tissues in different infection routes could reproduce the disease with mortality ranging from 56% to 84% at 5 days post inoculation.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
1999年第2期33-37,共5页
Journal of Yangzhou University：Natural Science Edition
基金
江苏省"九五"攻关项目
关键词
对虾
杆状病毒
病理学
暴发性流行病
中国
penaeid shrimp
pathology
baculovirus
explosive epidemic disease
artificial infection
