摘要
目的分析免疫性血管炎家兔血清8-异前列腺素F2α水平变化,探讨其在血管炎发病机制中的意义及N-乙酰半胱氨酸(N-acetylcysteine,NAC)治疗对其的影响。方法日本大耳幼年家兔60只分为正常对照组、模型对照组、NAC治疗组,用牛血清白蛋白复制幼兔免疫性血管炎的川崎病(KD)动物模型,治疗第7天及第28天检测各组血清8-异前列腺素F2α水平、苏木精-伊红染色观察其血管病变。结果免疫性血管炎家兔急性期血清8-异前列腺素F2α水平较正常对照组显著升高(P<0.05),NAC可显著降低血清8-异前列腺素F2α水平(P<0.05),并能显著改善免疫性血管炎家兔的血管病理损害。恢复期模型对照组幼兔血清8-异前列腺素F2α水平仍显著高于正常对照组(P<0.05)。结论血清8-异前列腺素F2α可能参与了免疫性血管炎的发病机制,NAC可能通过清除自由基来对抗免疫性血管炎。
Objective To analyze the change of serum 8-iso-prostaglandin F2α （8-iso-PGF2α） level for exploring its significance in the pathogenesis of immune vasculitis, and observe the effect of N-acetylcysteine （NAC） on the level of serum 8-iso-PGF2α in rabbits with immune vasculitis. Methods Sixty young rabbits were randomly divided into 3 groups, and consisting of the normal control group, the model control group, and the NAC group. An experimental model of weanling rabbits for Kawasaki disease （KD） was reproduced by the intravenous injection of bovine serum albumin. The serum level of 8-iso-PGF2α and the pathological changes of immune vasculitis were analyzed at day 7 and day 28 after the treatment of NAC. Results The serum level of 8-iso-PGF2α at acute phase was significantly higher in the model control group than that in the normal control group （P 〈 0.05）. NAC treatment could decrease the serum level of 8-iso-PGF2α and improve the vessels damage of immune vasculitis. The serum level of 8-iso-PGF2α at convalescent period remained higher in the model control group than that in the normal control group. Conclusions The serum 8-iso-PGF2α may be involved in the pathogenesis of immune vasculitis, NAC may protect against immune vasculitis by its free radical scavenging activity.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2011年第6期567-571,共5页
Journal of Clinical Pediatrics
基金
湖南省教育厅科研基金资助项目(No.07C571)
湖南省科技厅科研基金资助项目(No.2008SK3061)
