摘要
目的 探讨肺组织中氧化及抗氧化系统在高氧致早产儿慢性肺疾病 (CLD)中的动态变化规律。 方法 采用高浓度氧致早产鼠CLD模型为研究对象 ,分为实验组和对照组 ,各 4 0只。应用分光光度计比色法分别测定肺组织中脂质过氧化产物丙二醛 (MDA)的含量及超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶 (GSH Px)和过氧化氢酶 (CAT)的活性。 结果 实验组和对照组各时间点肺组织中SOD、GSH Px及CAT的活性差异均无显著性 (P >0 .0 5 ) ;而实验组MDA水平早期即明显升高 [(5 5 .92± 5 .5 3)nmol/mg肺组织 ,P <0 .0 1],7d达高峰 [(94 .30± 12 .4 0 )nmol/mg肺组织 ,P <0 .0 0 1],持续 1周后逐渐下降 ,2 1d时仍高于正常水平 [(47.95± 7.4 8)nmol/mg肺组织 ,P <0 .0 1]。 结论 肺组织中抗氧化酶系统的活性不足及氧自由基损伤始终伴随高氧致CLD发生、发展的全过程。
Objective To investigate the changes of oxidative and antioxidative system of the lung tissue in hyperoxia induced chronic lung disease (CLD). Methods The CLD rat model was established under hyperoxic exposure and then divided into experimental and control group. Malondialdehyde (MDA) concentration and the activity of superoxide dismutase(SOD), glutathione peroxidase(GSH Px)and catalase(CAT) were measured. Results The activity of SOD, GSH Px and CAT in both experimental and control groups did not show any significant difference( P >0.05). However, the MDA level in the experimental group was higher than that of the control on day 3 [(55.92±5.53)nmol/mg prot vs (22.52±4.36)nmol/mg prot, P <0.01], reached the peak level on day 7 [(94.30± 12.40) nmol/mg prot vs (24.27±5.48)nmol/mg prot, P <0.001] and then decreased gradually but remained at a higher level than control group on day 21 [(47.95±7.48)nmol/mg prot vs (23.57± 5.67) nmol/mg prot, P <0.01]. Conclusion Deficiency of antioxidant enzyme and injury of oxygenic free radical in pulmonary tissue accompanies the process of hyperoxia induced CLD.
出处
《中华围产医学杂志》
CAS
2004年第6期361-363,共3页
Chinese Journal of Perinatal Medicine
