摘要
经硫酸铵沉淀、Bio Rex70树脂的阳离子交换层析和MonoQ预装柱的阴离子交换层析 ,从大鼠急性期血清中分离纯化了脂多糖结合蛋白 .SDS PAGE为单一条带 ,分子量 60kD ,所纯化的大鼠脂多糖结合蛋白可明显增强脂多糖与单核巨噬细胞的结合 .脂多糖结合蛋白对脂多糖诱导肺泡巨噬细胞中肿瘤坏死因子α(TNF α)mRNA表达的调节作用具有明显的剂量依赖性 .
The lipopolysaccharide\|binding protein form actue phase rat serum was purified by precipitation of ammonium sulphate,Bio\|Rex70 resin and MonoQ column. SDS\|PAGE analysis indicated that the purified preparation of rat lipopolysaccharide\|binding protein showed homogeneity and had molecular weight of 60 kD.The binding of lipopolysaccharide to mononuclear cells or macrophages were enhanced by purified lipopolysaccharide\|binding protein and the regulatory effect of lipopolysaccharide\|binding protein on TNF\| α mRNA expression induced by lipopolysaccharide in alveolar macrophage was dose dependent.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2001年第5期647-650,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金重点资助项目 ( 3 973 0 2 0 1)
