摘要
本文运用随机扩增多态性ＤＮＡ（ＲＡＰＤ）标记方法，从ＤＭＡ水平对小麦异交群体４个子群体的遗传结构进行了研究．１０个引物共检测６５个位点，研究结果表明：（１）各子群体均保持了丰富的遗传变异；（２）连续五次选择对千粒重选重子群体的遗传结构影响不大；而在株高选低子群体中，随着选择次数的增加，群体的遗传变异有逐渐减小的趋势．（３）子群体间已出现一定分化但大部分遗传变异仍存在于子群体内部；其中，不同选择处理群体间的遗传距离较大，已发生了比较明显的分化；同一选择处理群体，选择次数越多，群体分化程度越大，另外，在本研究数据处理中，首次将ＲＡＰＤ谱带结果转化为基因频率分析群体遗传结构。
The genetic constitution of the out breeding populations in winter wheat was studied at DNA level with the RAPD(random amplified polymorphic DNA)method.RAPD polymorphism was examined in four subpopulations.Certain region of the whole genome was covered by 65 locies examined with 10 random primers.The results showed that:(1)Each subpopulation maintained sufficient genetic polymorphism;(2)The genetic constitution of the subpopulation selected for heavey kernel weight(W5) didn′t change greatly after 5 generations of successive selection.However,in the subpopulations for short plant,with the increasing of selecting generations,the genetic variation within the population decreased gradually;(3)Some genetic differentiation expressed among subpopulations,but most variation still existed within subpopulations.The genetic differentiation between the different selecting subpopulations was significant.In the subpopulations for short plant,with the increasing of selecting generations,the differentiation increased correspondly.In addition,by translating RAPD patterns to gene frequences,the genetic constitution of the populations was analysed.The results were all in agreement with other analysis methods.
出处
《首都师范大学学报（自然科学版）》
1999年第3期53-61,共9页
Journal of Capital Normal University：Natural Science Edition
基金
国家自然科学基金
关键词
小麦异交群体
遗传结构
RAPD分析
育种
wheat outbreeding population,genetic constitution,RAPD analysis.
