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大豆卵磷脂中脑苷脂类物质的分析与检测研究 被引量：2

Study on Analysis and Detection of Cerebrosides from Soybean Lecithin
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摘要 通过电喷雾质谱(ESI/MS)和高效液相色谱-蒸发光散射器(HPLC-ELSD)鉴定和分析用柱层析法纯化大豆卵磷脂得到的各组分主要成分。经质谱检测纯化的组分中主要有两种分子类型的神经酰胺,分别是C18:0 d18:1Cer和C25:0 h d18:1Cer;主要有两种分子类型的脑苷脂,分别是C16:0 t18:2Ce和C17:0Ce。通过HPLC-ELSD分析,二次柱层析得到的神经酰胺含量99.43%,回收率为98.23%;脑苷脂含量为98.86%,回收率为97.27%。 The composition of portions purified from soy lecithin by silica gel column chromatography （SGCC） was analyzed using ESI/MS （electrospray ionization mass spectrometry） and HPLC -ELSD （evaporative light scattering detector）. Two molecular species of ceramide （Cer） （ C18：0 d18：1 Cer and C25 ：Oh d18：1 C er） and two molecular species of cerebroside （CE） （C16：0 tl8：2Ce and C17：0Ce） were identified by ESI/MS in the portions. A content of 99.43% with a recovery of 98.23% and a content of 98.86% with a recovery of 97.27% were detected from portions of the second SGCC by HPLC-ELSD for Cer and CE, respectively.
作者 韩雪 汪勇 胡长鹰 王丽丽 刘元法 王兴国
机构地区 暨南大学食品科学与工程系 江南大学食品学院
出处 《中国粮油学报》 EI CAS CSCD 北大核心 2011年第5期111-118,共8页 Journal of the Chinese Cereals and Oils Association
基金 "863"计划(2010AA101505) 中央高校基本科研业务费专项资金(21610501)
关键词 脑苷脂 神经酰胺 电喷雾质谱 HPLC-ELSD cerebroside, ceramides, ESI/MS, HPLC - ELSD
分类号 TS227 [轻工技术与工程—粮食、油脂及植物蛋白工程]
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参考文献19

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二级参考文献16

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共引文献17

同被引文献22

引证文献2

二级引证文献7

中国粮油学报
2011年 第5期

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