摘要
采用超高压液相色谱-串联四级杆质谱仪（UPLC-MS/MS）（配有电喷雾离子源）法检测磷脂酰胆碱（PC）,先使用气相色谱分析可能包含的PC分子种类,再根据气相色谱分析结果,UPLC-MS/MS进行准确的定性和定量。UPLC的流动相：A相为水,B相为乙腈∶甲醇=5∶5,A∶B=10∶90,流速为0.4mL/min,PC大约在4min时出峰。最低检出限为1μg/L,相关系数为0.99936,加标回收率为95.6%~98.4%。
The phosphatidylcholine was detected with UPLC-MS/MS（equipped with electrospray ionization source）. Gas chromatography（GC） was used to analysis PC molecular species,according to the results of GC,and then UPLC-MS/MS be used to accurate qualitative and quantitative. The mobile phase of UPLC=A, water. B,acetonitrile ：methanol =5：5,A ：B =10：90, had flow rate of 0.4mL/min. Under this condition,the retention time of PC was about 4min. The regression coefficient was 0.99936 and the limit of detection was 1ug/L. The recovery was in the range of 95.6%-98.4%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第16期96-98,共3页
Science and Technology of Food Industry
关键词
超高压液相色谱
电喷雾质谱
气相色谱
磷脂酰胆碱
Ultra Performance LC （ UPLC ）
electrospray ionization mass spectrometry
gas chromatography
phosphatidylcholine
