摘要
分别用细胞松驰素 Ｂ、咖啡因、６二甲基氨基嘌呤处理皱纹盘鲍（ Ｈａｌｉｏｔｉｓ ｄｉｓｃｕｓ Ｈａｎｎａｉ） 受精卵抑制第一极体后，分析了对照组和处理组受精卵染色体的分离情况。结果表明：对照组受精卵具１８ 条四分体染色体，经过减Ⅰ和减Ⅱ，放出两个极体，受精卵的发育具有不同步性；处理组受精卵在第二次减数分裂中出现了特殊的分离类型，即“三极分离”、“联合二级分离”、“独立二极分离”。分析了三种药物抑制第一极体的机理及效果。
Fertilizated eggs of abalone \%Haliotis discus\% Hannai were treated with cytochalasin B, cafeine and 6 dimethylaminoparine (6 DMAP) to block polar body Ⅰ, and the separated chromosomes of control and experimental groups were analysed. The results shows that the fertilized egg in the control group has 18 chromosomes and extrude 2 polar bodys after meiosis Ⅰ and Ⅱ. The development of fertilized egg was remarkably asynchronous. The special types of chromosome segregation were observed in the treated groups during meiosis Ⅱ i.e.: tripolar segregation, united bipolar segregation and separated bipolar segregation. The paper preliminarily analyzed mechanism and effect of the 3 types medicine on blocking PBI.
出处
《大连水产学院学报》
CSCD
1999年第3期7-12,共6页
Journal of Dalian Fisheries University
基金
国家"863"鲍多倍体育种技术研究课题
关键词
皱纹盘鲍
诱导
三倍体
细胞松驰素B
咖啡因
Haliotis discus\% Hannai
inducing
triploid
cytochalasin B
cafeine
6 dinethylaminoparine
chromosome
segregation
