摘要
采用AFLP标记技术结合"拟测交"策略,对泥蚶父、母本和109个F1全同胞个体进行了遗传连锁的分析研究,构建了泥蚶的遗传连锁图谱。结果表明,在638个分离标记中,符合1:1孟德尔分离类型位点的母本和父本分离标记分别为235和259个;3:1孟德尔分离类型位点59个。雌性图谱包括21个连锁群,标记间平均间隔为8.12cM,图谱共覆盖1737.8cM,覆盖率为83.1%;雄性图谱包括20个连锁群,标记间平均间隔为5.81cM,图谱共覆盖1388.2cM,覆盖率为84.7%。
Based on segregation of amplified fragment length polymorphism （AFLP） markers, the first genetic linkage map of Tegillarca granosa Linnaeus was constructed using the “two-way pseudo-testcross” strategy. A segregation popula- tion （F1） including 109 progenies （full-sib family） from the cross between two wild populations of T. granosa from Jiangsu and Yueqing was obtained. The parents and the F1 progeny were used as the segregation population. A set of 66-pair primer combination were used to analyze the parents and the 109 progenies of the mapping family. A total of 638 segregation sites were identified. Among them, 494 （235 for female and 259 for male） fitted in the expected 1 ： 1 Mendelian segregation ratio and 59 fitted in a 3 ： 1 Mendelian ration. The female linkage maps included 21 linkage groups, spanning 1737.8cM with the average marker density of 8.12cM, and the observed coverage was 83.1%. The male linkage maps included 20 linkage groups, spanning 1388.2cM with the average marker density of 5.81cM, and the observed coverage was 84.7%.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2012年第5期983-991,共9页
Oceanologia Et Limnologia Sinica
基金
国家高技术研究发展计划(863计划)
2012AA10A410-1号
浙江省自然科学基金项目
Y3110003
浙江省科研院所专项
2009F20010号
温州市科技兴海项目
S20080019号
关键词
泥蚶
AFLP
遗传连锁图谱
拟测交策略
Tegillarca granosa, AFLP, Genetic linkage map, Double pseudo-testcross strategy
