摘要
本实验用细胞松驰素B(CB)抑制栉孔扇贝Chlamys(Azumapecten) farreri(Jones & Preston)第一极体的排出产生三倍体。抑制第一极体排出的最佳时间是在卵子受精后10min。25℃下,卵子受精后10min,0.05ppm的CB持续处理20min,获得了50％的三倍体。23℃下,卵子受精后10min,0.1ppm的CB持续处理15min,得到了39％的三倍体。
In this experiment, Cytochalasin B was used to induce 1 pb-triploidy in Chlamys farreri(Jones and Prestion). The best time of inhibition of the first polar body is 10 min after fertilization. At 25℃, treatment for 20 min duration 10 min after fertilization resulted in 50% triploidy of scallops in the 0.05mg CB/0.1 ml DMSO/L sea water Cytochalasin B treatment group. At 23℃, treatment for 15 min duration 10 min after fertilization resulted in 39% triploidy of scallops in the 0.1 mg CB/0.1ml DMSO/L sea water Cytochalasin B treatment group.
出处
《海洋湖沼通报》
CSCD
北大核心
1992年第2期40-45,共6页
Transactions of Oceanology and Limnology
关键词
细胞松驰素B
栉孔扇贝
三倍体
Cytochalasin B
Scallop
Chlamys farreri(Jones & Proston)
Triploidy
