摘要
目的研究大蒜素对尼古丁所致人牙周膜成纤维细胞(HPDLCs)氧化毒性的保护作用。方法 1)建立尼古丁对HPDLCs氧化毒性的模型,采用水溶性四氮唑(WST)比色法选出可有效抑制HPDLCs存活率的尼古丁质量浓度X,用于后续实验。2)将体外培养的第5代HPDLCs分为空白组、尼古丁组、尼古丁+大蒜素组(大蒜素质量浓度分别为15、30、60μg·mL-1),以不同培养液培养24 h后,采用WST比色法选出可显著改善HPDLCs存活率的大蒜素质量浓度Y用于后续实验。3)将体外培养的第5代HPDLCs分为空白组、尼古丁组、尼古丁+大蒜素组,分别于培养1、4、8、12、24 h后测量细胞内谷胱甘肽(GSH)浓度。结果 0.8 mg·mL-1尼古丁即可显著抑制HPDLCs存活率(细胞存活率为空白组的77%,P<0.05,取X为0.8 mg.mL-1);而60μg·mL-1大蒜素可显著改善此负性作用,将细胞存活率提升至尼古丁组的112%(P<0.05),且与空白组比较无统计学差异(P>0.05),取Y为60μg·mL-1。在培养后的各时间点,尼古丁+大蒜素组GSH浓度与空白组相比均无统计学差异(P>0.05),但较尼古丁组均有明显提高(P<0.05);培养8 h时,尼古丁+大蒜素组GSH浓度与尼古丁组相比差异最大,前者较后者提高82%。结论 60μg·mL-1大蒜素可以显著降低尼古丁对HPDLCs的氧化损伤作用。
Objective To explore the protective effect of allicin on nicotine-induced oxidative damage to human periodontal ligament cells（HPDLCs）.Methods 1）Establish nicotine-induced oxidative damage model on HPDLCs. Use water-soluble tetrazolium（WST） colorimetric method to find out the nicotine concentration（X） that could inhibit HPDLCs＇growth for the following experiments.2）HPDLCs of the fifth passage were divided into 5 groups：The control group,the nicotine group and the nicotine＋allicin groups（the concentration of allicin was 15,30,and 60 μg·mL-1 respectively）.Different kinds of culture media were added.Similarly,use WST colorimetric method to choose the allicin concentration（Y） that could significantly improve the survival rate of HPDLCs.3）HPDLCs were divided into 3 groups：The control group,the nicotine group,the nicotine＋allicin group and different media were added.The glutathion（GSH） concentrations in HPDLCs were determined in 1,4,8,12 and 24 h respectively.Results 0.8 mg·mL-1 nicotine could inhibit the HPDLCs survival rate significantly（77% of the control,P0.05）.But 60 μg·mL-1 allicin could prevent the inhibition effects evidently,improving the survival rate to 112% of that of the nicotine group（P0.05） and reaching the survival rate level of control group（P0.05）.The GSH concentrations of nicotine＋allicin group were higher than that of the nicotine group always（P0.05） and by 82% at 8 h after culture,but had no difference with that of the control group（P0.05）.Conclusion 60 μg·mL-1 allicin can protect the HPDLCs against oxidative damage induced by nicotine.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2011年第1期9-12,共4页
West China Journal of Stomatology
基金
湖南省科技厅基金资助项目(2010FJ3067)
中南大学理科发展基金资助项目(1773-206001081)
