摘要
葡萄糖氧化酶是氧化葡萄糖的专一催化酶,具有脱氧、杀菌的功能。通过定性和定量抗菌实验方法研究了葡萄糖/葡萄糖氧化酶体系在以淀粉作为载体和不混合淀粉时施涂于纸张表面的抗菌效果。定性抗菌实验表明,葡萄糖/葡萄糖氧化酶抗菌纸具有良好的抗菌效果。而定量检测结果表明,pH等于5.5时,35℃反应10min,不添加淀粉时,葡萄糖氧化酶最小的抑菌浓度约为70U/m2,葡萄糖足量时,酶用量越高,抗菌率越好;添加0.7g/m2淀粉后,最小抑菌浓度提高到约650U/m2。淀粉对葡萄糖/葡萄糖氧化酶体系的抗菌效果有较大影响,主要原因是淀粉能够促进细菌的繁殖。
Glucose oxidase is specific for β-D-glucose, and of oxygen removal and antibacterial functions. Antimicrobial effects of paper coated via glucose/glucose oxidase with/without starch were investigated by means of qualitative and quantitative assay. Qualitative assay phenomenon showed that glucose/enzyme paper was capable of inhibiting bacteria growth to better extent. The experimental results from quantitative assay demonstrated that minimal inhibition concentration（MIC） was about 70U per square meter under the conditions of pH 5.5,35 centigrade, 10 minutes reaction time,and without starch.And that if the dosage level of enzyme rises with saturation of glucose,the germproof effects could be more remarkable. However, MIC was up to round 650U per square meter with 0.7 gram per square meter starch addition.The starch hereby imposed a significant negative effect on the antibacterial properties of glucose/enzyme paper. This owes to starch nutriment contributing to bacteria growth.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第1期124-127,共4页
Science and Technology of Food Industry
关键词
葡萄糖/葡萄糖氧化酶
抗菌纸
抗菌性
定性与定量抗菌实验
glucose/glucose oxidase
antibacterial paper
antimicrobial property qualitative and quantitative assay
