摘要
以４种不同的Ｎ－长链烷酰－Ｌ－氨基酸胶束为手性选择剂，对３种不同性质的手性化合物（α－氯代丙酰替苯胺，２－氨基－３－对硝基苯基－１，２－丙二醇和华法林）的毛细管胶束电动色谱分离进行研究．结果表明，手性表面活性剂中不同的氨基酸残基和烷基链的长度对分离影响较大；随手性表面活性剂浓度增加，溶质保留时间增大，分离度增加，不同溶质的最佳分离浓度在１００～１５０ｍｍｏｌ／Ｌ之间；ｐＨ对电中性手性化合物分离影响不大，但对酸性或碱性手性化合物的分离影响较大．在选定的条件下，３种样品均在２０ｍｉｎ内完全分离。
Several synthetic chiral surfactants such as
Ndodecanoyl or NtetradecanoylLamino acids were used as the chiral selector respectively. The
neutral, acidic and basic enantiomers such as chloropropionanilide,
2amino3(4nitrophenyl)1,2propanediol and warfarin were separated by MEKC. The effects of the
type and concentration of chiral surfactants, pH of buffer solution on the enantiomeric
separation were investigated. The results showed that the side group of amino acid and the
chain length of hydrophobic alkyl group in chiral surfactant molecule had a significant influence
on the separation. The concentration of chiral surfactant was an important factor influencing the
enantiomeric separation. Both retention time and resolution increased with increasing the
concentration of chiral surfactant. The optimum concentration was in the range from 100 to 150
mmol/L for the test samples. The pH of buffer solution had a dramatic effect on separation of the
weak basic or acidic enantiomers, but little effect on neutral enantiomer. Under the selected
condition, several test enantiomers were successfully separated. The separation efficiency was
more than 1105 theoretical plates per meter.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第4期539-543,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
浙江省自然科学基金
关键词
电动色谱
手性分离
拆分
毛细管胶束
MEKC
Electrokinetic chromatography, Chiral
separation, Long chain NacylL\|amino acid, Micelles
