摘要
在自制的高效毛细管电泳装置上,使用内径为0.05毫米,长为450毫米的熔融石英毛细管,十二烷基硫酸钠胶束溶液,254nm检测波长,进行了电中性化合物的毛细管胶束电动色谱研究。并将乙醇-水混合溶剂引入体系,成功地进行了多环芳烃的分离。考察了电场强度,SDS浓度及乙醇含量对柱效的影响。
Micellar electrokinetic capillary chromatography (MECC) of electric neutral compounds wasstudied on a 0.05mm i.d. x 450mm fused silica capillary tube with sodium dodecyl sulfate(SDS)solution and 254nm UV detection. In open-tubular capillary two phases, aqueous and micellar,move with different velocities and micellar solubilization operates as the distribution process ofsolutes. A novel mixed ethanol-water micellar solution was introduced to MECC of weak polarcompounds. The effects of electric field strength, concentration of SDS and ethanol content oncolumn efficency were examined.
关键词
胶束增溶
电动色谱
毛细管
Micellar solubilization
micellar electrokinetic capillary chromatography
