摘要
建立了一种基于硫酸水解-高效液相色谱法测定低聚木糖产品中低聚木糖含量的简捷方法。经过优化实验,硫酸水解的条件:硫酸质量分数6.0%、反应温度100℃、反应时间60 min;高效液相色谱测定的条件:色谱柱B io-Rad Aminex HPX-87H、流动相5 mmol/L H2SO4、洗脱流速0.6 mL/min、柱温55℃,采用外标法和峰面积积分法测得样品中低聚木糖质量分数为39.94%,重复性好,标准差为0.36%。
A simple and convenient method on xylo-oligosaccharide product （XOS） quantitative determination was established based on dilute sulfuric acid hydrolysis combined with high performance liquid chromatography （SAH-HPLC） detection. The protocol is as following：on HPLC system, the xylose content of XOS, the standard xylose and its hydrolysates was determined, and then total XOS content was calculated according to its xylose content changes, the xylose decomposition ratio and the conversion ratio of XOS to xylose during SAH. The optimum conditions for SAH step is at 6.0 % sulfuric acid content and 100℃ for 60 min. The optimum conditions for HPLC detection step is at the lution rate of 0.6 mL/min with 0. 005 mol/L sulfuric acid by the column Bio-Rad Aminex HPX-87H under 55℃ , and the xylose content was quantitatively detected and calculated according to the external standard method and peak area integration. The repeatability and accuracy of the SAH-HPLC method was tested for some XOS product determination. The result showed the XOS content was 39.94 % in the product at a good repeatability and accuracy and the standard deviation of 0.36 %.
出处
《生物质化学工程》
CAS
2010年第6期14-17,共4页
Biomass Chemical Engineering
基金
国家自然科学基金(30871992)
江苏省自然科学基金(BK2009690)
关键词
低聚木糖
高效液相色谱法
稀硫酸水解
xylo-oligosaccharides
high performance liquid chromatography
dilute sulfuric acid hydrolysis
