摘要
以1株谷氨酸高产的谷氨酸棒状杆菌(Corynebacterium glutamicum)A-26为出发菌株,先后采用亚硝基胍(NTG),紫外线进行诱变,并选用谷氨酰胺的2种结构类似物磺胺胍(SG)、甲硫氨酸亚砜(MSO)以及(NH4)2SO4筛选出具有三重抗性标记的谷氨酰胺高产菌株C.glutamicum N-U-6,其谷氨酰胺产量可达33.54 g/L,比出发菌株的产量(15.64 g/L)高出1.14倍。并检验了该诱变菌株具有较好的遗传稳定性。
Glutamine is the most abundant amino acid in the blood and has the ability to help preserve the intra- cellular pool of free amino acids in the body, which is a regulator of protein synthesis and correlates with nitrogen bal- ance. Being the original strain, Corynebacterium glutamicum A-26 was mutagenized by NTG and the UV-radiation, then a mutant resistant to sulfaguanidine （SG） , methionine sulfoxide （MSO） and （NH4）2SO4 was obtained. It was named Corynebacterium glutamicu N-U-6. 1.14 times glutamine （33.54 g/L） more than the original strain （15.64 g/ L） was produced by the mutant. Its good hereditary stability was also tested.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2010年第8期64-67,共4页
Food and Fermentation Industries
基金
滨州学院青年人才创新工程基金项目(No.BZXYQNLG200607)及(No.BZXYQNLG200716)联合资助
关键词
L-谷氨酰胺
诱变
抗性
选育
L-glutamine, mutagenesis, resistance, screening
