摘要
用ｄ－生物素代替玉米浆的摇瓶发酵实验，筛选出高产酸菌株，测定了ｄ－生物素的亚运量可变范围。比较了单一玉米浆、单一ｄ－生物素与两者各半量发酵结果及不同接种量对产酸的影响。采用ｄ－生物素代替半量玉米浆的中糖发酵工艺应用于生产。结果证明此工艺可行，降低了成本，提高了产酸率，平均产酸率达７．８１％，平均转化率达５３．２％。
With the fermentative experiments of shake flask in which corn steep liquor was replaced by d-biotin,the high acid-produced strains were selected and the variable range of sub opportune amount of d-biotin was determined.By comparing the fermentative results in using single corn steep liquor,Singte d-biotin and both in half amount.and the effects of different inoculating amount on producing acid,the medium-sugar fermentative technology of substitution of d-biotin for half-amount corn steep liquor was used in production.This technology has been proved feasible by its decreasing cost and increasing acid-produced rate.Average acid-produced rate is 7.81 % and average transformation is 53.2 %.
出处
《生物技术》
CAS
CSCD
1996年第2期30-34,共5页
Biotechnology
关键词
谷氨酸
发酵
D-生物素
玉米浆
d-biotin
Corn steep liquor
Glutamic acid
Fermentation
