摘要
目的:建立芪参益智胶囊的质量标准。方法:采用薄层色谱法对芪参益智胶囊中党参、枸杞子、陈皮等进行定性鉴别;采用高效液相色谱法测定黄芪甲苷的含量。色谱柱:Agilent Hypersil ODS十八烷基键合硅胶柱(4.6 mm×250 mm,5μm);流动相乙腈-水(32∶68);流速1.0 mL·min-1;漂移管温度:45℃;气体流速1.5 L·min-1。结果:定性鉴别薄层色谱斑点清晰,阴性无干扰;黄芪甲苷在0.506～4.554μg呈良好线性关系;平均回收率为103.05%,RSD为1.43%(n=6)。结论:该方法简便、准确、可靠,可作为芪参益智胶囊的质量控制方法。
Objective： To establish the quality standard of Qishenyizhi Capsule.Method： TLC were used to distinguish Dangshen,Pugongying,Zhizi and Chishao.HPLC were used for the deterimination of Astragaloside Ⅳ.The column was PHENOMENEX ODS C18（4.6 mm ×250 mm,5 μm）;the acetonitrile-water（35∶65） was used as mobile phase,the flow rate was 1.0 mL·min-1,the temperature of drift tube was 45 ℃ and the flow rate of N2 was 1.5 L·min-1.Result： The thin-layer chromatography spots were clear and the negative samples have no interferences;and astragaloside Ⅳ was linear in the range of 0.506～4.554 μg,and the average recovery was 103.05% with RSD 1.43%（n=6）.Conclusion： This method is simple,accurate,reliable and can be used as a quality control method for Qishenyizhi Capsule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第12期80-82,共3页
Chinese Journal of Experimental Traditional Medical Formulae
