摘要
为探讨快速而准确的检测香蕉束顶病毒的方法，采用本所制备的香蕉束顶病毒（Ｂａｎａｎａｂｕｎｃｈｙｔｏｐｖｉｒｕｓ－ＢＢＴＶ）多克隆抗体ＩｇＧ和台湾的香蕉束顶病毒单克隆抗体２Ｈ６，用酶联免疫吸附测定技术（ＥＬＩＳＡ）双抗体夹心法（ＤＡＳ）测定了漳州市天宝的香蕉束顶病病株。试验结果表明，多抗和单抗相结合的ＤＡＳ－ＥＬＩＳＡ法能检测到病组织汁液的最大稀释度为１∶６４０，而单独使用单抗的最大稀释度为１∶１０。同时采用多抗和单抗相结合的ＤＡＳ－ＥＬＩＳＡ法比较了病株根、假茎、叶片中脉、叶肉的测定结果，假茎和中脉的病毒含量高于叶肉，根未检出。用此法能检测到蚜虫体内的病毒。
In order to rapidly and accurately detect banana bunchy top virus BBTV,the samples of plants infected by BBTV collected from Tianbao,Zhangzhou were identified by method of ELISA(Enzyme linked immunoadsorbent assays),using polyantibody IgG of BBTV prepared by this laboratory and monoantibody of BBTV from Taiwan respectively.The result showed that the maximum dilution of the liquid of infecting plant tissues was 1∶640 by combination of polyantibody and monoantibody.While using polyantibody alone,the maximum dilution of the liquid of infecting plant tissues was 1∶10.Compared with root,stem,main vein and leaf of infected plants by DAS ELISA of combination of poly and mono antibody showed that stem and main vein contained virus and virus in stem and main vein more than in leaf but could not detect virus in root.This method could be used to detect BBTV succesfully in aphides from an infective colony.
出处
《福建农业学报》
CAS
1999年第1期19-22,共4页
Fujian Journal of Agricultural Sciences
