摘要
目的研究p16和Ki-67蛋白在宫颈组织中的表达,探讨它们在宫颈病变中的诊断价值。方法应用免疫组化技术SP法检测正常宫颈或炎性病变组织20例、CINⅠ组织22例、CINⅡ组织20例、CINⅢ组织20例、宫颈腺体受累及组织20例(未见原发病灶)、宫颈鳞状细胞癌组织20例中p16和Ki-67蛋白的表达情况。结果 p16和Ki-67蛋白在正常宫颈组织中的阳性表达率明显低于宫颈癌前病变及宫颈癌组织,差异有显著意义(P<0.01)。Ki-67蛋白阳性表达在良性宫颈组织与CINⅠ组织相比较,无显著差异(P>0.05);p16阳性表达在宫颈基底细胞增生组织与CINⅠ组织相比较,有显著差异(P<0.05),而Ki-67蛋白无显著差异(P>0.05);p16着色强阳性在CINⅠ与CINⅡ、CINⅢ、宫颈癌比较,有显著差异(P<0.01)。结论 p16蛋白能很好地鉴别宫颈良性病变、宫颈上皮内瘤变以及宫颈癌;在鉴别宫颈基底细胞增生与CINⅠ时,p16能起辅助诊断作用;应用p16的着色强弱能够很好地区分CINⅠ与CINⅡ、CINⅢ,有助于CIN的判断与分级。Ki-67能很好地区分宫颈高度病变与宫颈癌,但不能区分宫颈良性病变与宫颈低度病变,需结合p16蛋白联合判断。
Objective To study the p16 and Ki-67 proteins in cervical carcinoma and to explore their potential in the diagnosis of cervical lesions. Methods Immunohistochemical technique was used to detect p16 and Ki-67 expression in 20 cases of normal or inflammatory cervical lesions, 22 eervical CIN Ⅰ , 20 cervical CIN Ⅱ, 20 cervical CIN Ⅲ, 20 cervical glandular dysplasia, and 20 cervial carcinoma. Results The positive expression rates of p16 and Ki-67 proteins in normal cervical tissue were significantly lower than the cervical precancerous lesions and cervical carcinoma, the difference were significant （P 〈 0. 01 ）. Ki-67 protein expressions in normal cervical tissue and CIN I tissues compared with no significant difference （ P 〉 0.05 ） ; p16 positive expression of basal cell hyperplasia in cervical tissue was compared with CIN Ⅰ , there was significant differences （ P 〈 0. 05 ） , while the Ki-67 protein with no significant difference （P 〉0. 05） ; p16 coloring strongly positive Among CIN Ⅰ ,CIN Ⅱ ,CIN Ⅲ , and cervical comparison, there were significant differences （ P 〈 0. 01 ）. Conclusion p16 protein can be good identification of benign cervix and cervical intraepithelial neoplasia and cervical cancer; identify basal cell hyperplasia of cervical organization and CIN Ⅰ organizations, p16 can play a role of diagnosis. Application of the coloring strength of p16 can be a very a good distinction between low-grade cervical lesions （ CIN Ⅰ ） and cervical lesion height （ CIN Ⅱ , CIN Ⅲ ） , which will help the judge and grade of CIN. Application of Ki-67 can be a good marker of cervical lesions and cervical cancer, a high degree, but can not mark the cervix benign lesions and low-grade cervical lesions, should be combined with p16 protein.
出处
《临床肿瘤学杂志》
CAS
2010年第7期613-616,共4页
Chinese Clinical Oncology
