摘要
目的：探讨食管癌变过程中肿瘤抑制基因ｐ５３和癌基因ｃ－ｍｙｃ、ｂｃｌ－２的变化及其与细胞凋亡的关系。方法：采用免疫组化法（ＡＢＣ）检测２７９例食管粘膜活检组织中ｐ５３、ｃ－ｍｙｃ、ｂｃｌ－２的表达以及细胞凋亡的变化。结果：从食管正常上皮到基底细胞增生、间变和癌，ｐ５３、ｃ－ｍｙｃ、ｂｃｌ－２免疫阳性表达率及细胞凋亡发生率和细胞凋亡指数（ＡＩ）均呈升高趋势，而且在同一阶段病变，ｐ５３和ｃ－ｍｙｃ阳性表达时凋亡指数高于其阴性表达，而ｂｃｌ－２阳性表达时凋亡指数低于其阴性表达。结论：在食管癌变过程中可能有多种肿瘤抑制基因和癌基因参与，细胞凋亡在食管癌变过程中可能有重要的生物学意义。
Purpose To investigate the relationship between the changes of tumor suppressor gene p53 and oncogenes cmyc, bcl2 and apoptosis in esophageal carcinogenesis. Methods Immunohistochemical method was carried out to detect the expression of p53, cmyc, bcl2 for 279 patients from esophageal endoscopic mucosa biopsies, and quantitive analysis for apoptosis was made. Results Immunoreactivity of p53, cmyc and bcl2, and occurrence of apoptosis and apoptotic index (AI)(the number of apoptotic cells and apoptotic bodies/mm2) showed increase from esophageal normal epithelia to basal cell hyperplasia and dysplasia and squamous cell carcinoma, and in the similar lesions p53 and cmyc positive lesions showed slightly higher AI than p53 and cmyc negative lesions. However, bcl2 positive lesions had lower AI than bcl2 negative lesions. Conclusions Multiple genes may involve in and apoptosis may have an important biological significance in esophageal carcinogenesis.
出处
《临床与实验病理学杂志》
CAS
CSCD
1998年第2期106-108,I017,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
河南省杰出青年科学基金
国家教委优秀青年教师基金
