摘要
目的:建立同时测定北五味子中五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素含量的方法。方法:以Symmetry(C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水,梯度洗脱,0～36 min(60∶40～66∶34);36～65min(66∶34～80∶20);65～70 min(80∶20),70～75 min(80∶20～100∶0),流速0.5 mL.min-1,检测波长254 nm。结果:五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素和五味子乙素分别在0.021～4.160,0.020～4.000,0.021～4.240,0.020～3.960,0.021～4.200μg呈良好的线性关系(r=0.999 9);平均加样回收率分别为104.8%,104.2%,102.7%,104.6%,104.5%。RSD值均小于3%。结论:本方法稳定易行、重复性好,可用于北五味子药材的质量评价。
Objective： To develop a HPLC quantitative method for determination of schisandrin,schisandrol B,schisantherin A,deoxyschisandrin and γ-schisandrin in Fructus Schisandrae.Method： A symmetry C18 column（4.6 mm × 250 mm,5 μm） was used with methanol（A） and water（B） as mobile phases,in gradient elution.The gradient program was as follows： 0-36 min,changed from 60%A to 66%A,36-65 min,to 80%A,65-70 min,kept for 80%A,70-75 min,to 100%A.The flow rate was 0.5 mL·min-1 and detection wavelength was set at 254 nm.Result： The linearities of schisandrin,schisandrol B,schisantherin A,deoxyschisandrin and γ-schisandrin were in the ranges of 0.021-4.160（r=0.999 9）,0.020-4.000（r=0.999 9）,0.021-4.240（r=0.999 9）,0.020-3.960（r=0.999 9） and 0.021-4.200（r=0.999 9）.The average recoveries were 104.8%,104.2%,102.7%,104.6%,104.5%,respectively.Conclusion： The method developed in this study was reliable,and can be used for the quality control of the fruits of S.chinensis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第13期1728-1730,共3页
China Journal of Chinese Materia Medica
基金
辽宁省重点项目(2007207002)
