摘要
目的测定不同产地醋五味子中五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素6种木脂素的含量。方法色谱柱为依利特ODS-C18(250mm×4.6mm,5μm);流动相为乙腈(A)-水(B),梯度洗脱(0～17min,A为50%;17～25min,A为50%～55%;25～30min,A为55%～75%;30～35min,A为75%;35～40min,A为75%～65%;40～45min,A为65%～50%)。结果 6种被测木脂素成分分离度良好;各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(r>0.999 5);重现性良好;准确度(RSD)分别为101.8%(1.5%)、99.5%(1.2%)、103.26%(2.1%)、99.8%(1.0%)、100.0%(1.9%)和101.3%(1.9%)。结论 HPLC法同时检测五味子6种木脂素成分,所建立方法稳定、可行。
OBJECTIVE To evaluate the quality of Schisandra vinegar products from different habitats by determining the contents of Schisandrin,Schisandrol B,Schisantherin A,Deoxyschisandrin,Schisandrin B and Schisandrin C.METHODS Elite ODS-C18 column（250 mm×4.6 mm,5 μm）was used.Acetonitrile（A）-water（B） was used as gradient mobile phase（0～17 min,50% A;17～25min,50% A→55% A;25～30min,55% A→75% A;30～35min,75% A;35～40min,75% A→65% A;40～45min,65% A→50% A）.The flow rate was 1.0 mL/min.The column temperature was 30 ℃ and the detection wavelength was 217 nm.RESULTS Excellent chromatographic separation was achieved for six studied lignans with good linearity（r〉0.999 5） over the studied concentration ranges.The average extraction recoveries（RSD）（n=9） were for Schisandrin,for Schisandrol B,for Schisantherrin A,for Deoxyschisandrin,for Schisandrin B and for Schisandrin C.CONCLUSION The HPLC method developed for simultaneous determination of six lignans is simple and valid.It provides the science basis for quality evaluation of Schisandra chinensis（Turcz.） Baill.and its processing products.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2012年第3期272-274,共3页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省教育厅重大项目(09KJA360001)
江苏省针药结合重点实验室课题(KJA200906)
