摘要
目的观察3木脂素类成分:异型南五味子丁素(heteroclitin D,HD)、五味子酚(schisanhenol,SAL)和(+)-安五脂素[(+)-anwulignan,AN]对血小板聚集的影响.方法采用Born比浊法,观察此3种木脂素在0.05～25 mg/L时对腺苷二磷酸(adenosine diphosphate,ADP)和血小板活化因子(platelet activating factor,PAF)诱导的兔血小板1、3、5 min聚集率和最大聚集率以及最大聚集时间的影响.结果①HD和SAL可呈剂量依赖性抑制ADP和PAF诱导的血小板最大聚集率,AN的抑制作用较弱.②对ADP诱导的血小板1、3、5 min聚集率,HD和SAL均可全程稳定抑制,AN 5mg/L在3、5min时有较明显的抑制作用;对PAF诱导的血小板1、3、5 min聚集率,HD在1、3 min时抑制作用较强,SAL在3、5 min时抑制作用较强.③HD、SAL和AN对ADP和PAF诱导的血小板最大聚集时间无明显影响.结论HD、SAL和AN在离体水平可不同程度抑制ADP和PAF诱导的兔血小板聚集.HD的抑制作用最强,可视为南五味子属药用植物的重要活性成分之一.
Purpose To investigate the effects of three lignans:heteroclitin D (HD),schisanhenol (SAL) and (+)-anwulignan(AN)with different skeletons from Schisandraceae medicinal plants on platelet aggregation. Methods At concentrations 0.05～25 mg/L of the three lignans, rabbit platelet aggregation induced by adenosine diphosphate (ADP) and platelet activating factor(PAF) were studied by Born's turbidimetry,the maximum time of platelet aggregation and platelet aggregation at 1,3,5 min were also observed. Results ① HD and SAL inhibited the ADP- and PAF-induced platelet aggregation in a concentration-dependant manner.The inhibitory effect of AN was weak.② ADP-induced platelet aggregation at 1,3,5 min were inhibited by HD and SAL in a similar manner.At 5 mg/L,AN inhibited platelet aggregation at 3 and 5 min significantly;The PAF-induced platelet aggregation at 1,3,5 min,the inhibition of HD at 1,3 min and of SAL at 3,5 min were more potent.③ HD,SAL and AN had no effects on maximum time of ADP- and PAF-induced platelet aggregation. Conclusions HD,SAL and AN have inhibitory effects on platelets aggregation induced by ADP and PAF in vitro.The inhibitory effect of HD is the most potent and could be one of the important active components from Kadsura medicinal plants.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2005年第4期467-470,478,共5页
Fudan University Journal of Medical Sciences
基金
教育部高校优秀青年教师教学与科研奖励基金项目(1999-71)
国家自然科学基金项目(32071586)
