摘要
目的观察４月龄和７月龄胎肝提取物（Ｅ４，Ｅ７）对ＢＥＬ７４０２肝癌细胞增殖和分化的影响．方法采用显微镜下细胞计数，３ＨＴｄＲ掺入和ＭＴＴ比色法，观察（１０～１０００）ｍｇ／Ｌ的Ｅ４和Ｅ７对培养中ＢＥＬ７４０２肝癌细胞的影响．结果Ｅ４从１００ｍｇ／Ｌ起可明显抑制７４０２细胞的生长和ＤＮＡ合成，细胞生长抑制ＩＣ５０为１４４４ｍｇ／Ｌ，ＤＮＡ合成抑制ＩＣ５０为２２７９ｍｇ／Ｌ．在（２５０～１０００）ｍｇ／Ｌ时对整体细胞线粒体脱氢酶活力有明显的抑制作用，ＩＣ５０为１０１３０ｍｇ／Ｌ．Ｅ７在（１０～１００）ｍｇ／Ｌ对细胞生长和ＤＮＡ合成具有明显的促进作用，至１０００ｍｇ／Ｌ浓度未发现明显的抑制作用．在５００ｍｇ／Ｌ和１０００ｍｇ／Ｌ浓度时对整体线粒体脱氢酶活力具有一定的抑制作用．对细胞生长曲线影响，Ｅ４表现为５００ｍｇ／Ｌ时能明显抑制７４０２细胞的生长，而在５０ｍｇ／Ｌ时影响不明显；Ｅ７表现为５００ｍｇ／Ｌ时影响不明显，而５０ｍｇ／Ｌ时则有明显的促进作用．（１００～１０００）ｍｇ／Ｌ的Ｅ４能明显促进单个细胞脱氢酶的活力，而Ｅ７只在１０００ｍｇ／Ｌ时有一定的促进作用，在（１０～５０）ｍｇ／Ｌ对额定细胞脱氢酶?
AIM To investigate the effect of the hepatic extracts (E 4,E 7) from 4 and 7 month old embryos on BEL 7402 hepatoma cell proliferation and differentiation in vitro . METHODS The effects of (10-1000) mg/L E 4 and E 7 on BEL 7402 hepatoma cells in culture were investigated by cell counting under microscope, 3 H TdR incorporation and MTT colorimetric assay. RESULTS E 4 obviously inhibited the proliferation and DNA synthesis of BEL 7402 hepatoma cells at (100-1000) mg/L concentrations, their half inhibitory concentrations (IC 50 ) were 144 4mg/L and 227 9mg/L respectively. E 4 also inhibited the total activities of mitochondrial dehydrogenase at (250-1000) mg/L ,the IC 50 was 1013 0mg/L . E 7 evidently promoted the proliferation and DNA synthesis of the cells at (10-100) mg/L ,and had no remarkable inhibitory effects until up to 1000mg/L , and also inhibited the total activities of dehydrogenase at (500-1000) mg/L . E 4 inhibited significantly the cell growth curve at 500mg/L and had no apparent effects at 50mg/L ;while E 7 had no remarkable effect on the cell growth curve at 500mg/L , and showed an obviously promoting effect at 50mg/L . E 4 at (100-1000) mg/L apparently promoted the dehydrogenase activities of a single cell. Only at 1000mg/L did E 7 have such promoting effects, at (10-50) mg/L E 7 had an obviously inhibitory effect on the mitochondrial dehydrogenase activity of definite number of hepatoma cells. CONCLUSION The embryonic cells express more differential factors to fit their differentiation at the early period, and cells produce more growth factors to fit their rapid proliferation at the late period.
出处
《世界华人消化杂志》
CAS
1999年第3期243-245,共3页
World Chinese Journal of Digestology
