摘要
本研究采用聚丙烯酰胺凝胶电泳和细胞体外培养方法,观察酶消化后人羊膜基膜的成分,以及它能否支持鸡胚前脑神经细胞的生长。肝素酶Ⅱ消化后的人羊膜基膜,对前脑神经元生长的支持作用,比未消化的基膜增强150％。电泳分析的结果表明,肝素酶Ⅱ消化后,类胶原多肽2α_1(260kD)和2α_2(225kD)减少,类基板蛋白多肽(212kD和200kD)增多;而经肝素酶Ⅰ、胶原酶Ⅰ消化后,基板蛋白减少。本文提示,基板蛋白是人羊膜基膜中促鸡胚前脑神经细胞生长的主要成分。
Cell culture and SDS-polyacrylamide gel electrophoresis (PAGE) methods were employed to analyse the role of human amniotic basement membrane (HABM) in supporting the growth of forebrain neurons and the components of HABM after enzymatic digestion. It was found that HABM treated with heparinase Ⅱ supported the growth of forebrain neurons 150% of that of the untreated HABM control. The results of SDS-PAGE analysis showed that HABM treated with heparinase Ⅱ had collagen-like polypeptides 2α_1 (260kD) and 2α_2 (225kD) reduced and lamininlike peptides (212kD and 200kD) increased; and that HABM treated with heparinase I or collagenase I had laminin reduced. This indicated that laminin is the major component in HABM to promote the growth of forebrain neurons.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第3期290-294,共5页
Acta Anatomica Sinica
基金
Croucher基金
京港学术交流中心
香港大学和医学院研究基金~~
关键词
人羊膜基膜
酶消化
前脑神经元
SDS-polyacrylamide gel electrophoresis
Human amniotic basement membrane
Enzymatic digestion
Forebrain neurons
Laminin
