摘要
从兔网织红细胞提纯的红细胞分化调节因子(erythroid differentiation factor,EDF),能对体外培养的自发转化成纤维细胞系L929及人鼻咽癌细胞系KB产生作用。当EDF剂量为0.10μg/ml时,可引起L929细胞形态发生改变,并有细胞核固缩现象。第2d的细胞生长抑制率为64.86％,软琼脂集落形成率为0;第5d时细胞增殖为负值。~3H-TdR掺入率明显降低。EDF剂量为0.15μg/ml时,对KB细胞生长已有抑制作用。EDF剂量达0.30μg/ml时,生长抑制明显。以上结果证明了EDF对恶性细胞具有增殖抑制作用。这种作用对不同种类细胞敏感性不同,并且与剂量呈正相关。
The inhibition of erythroid differentiation factor (EDF), which was extracted and purified from rabbit reticulocytes, on the growth of the mouse established fibroblast cell line L929 and human nasopharyngeal cell line KB was analysed in vitro. After addition of 0.10 μg/ml EDF to the culture media the shape of L929 cells became rounded and their nuclei were pyknotic. The inhibitory rate was 64.86% (day 2), for L929 cell the colony forming ability in soft agar decreased to zero. The incorporation rates of ~3H-TdR also decreased rapidly and the rates of cell proliferation changes to minus on 5th day. As for KB cells, it had an inhibitory effect when the concentration of EDF was 0.15μg/ml and it became obvious when its concenteation increased to 0.3μg/ml. All the results above shows that EDF has an inhibitory effect on the proliferation of tumor cells and the effect is cell type dependent as well as dose-dependent.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第3期285-289,共5页
Acta Anatomica Sinica
关键词
细胞增殖
细胞分化
L929细胞
Reticulocyte
Cell proliferation
Cell differentiation
L929 cell
KB cell
