摘要
目的:研究了异烟肼(Isoniazid,INH)在10-甲基吩噻嗪修饰碳糊电极(10-methylphenothiazine modified carbon paste e-lectrode,MPT/CPE)上的电化学行为和电化学分析方法。方法:循环伏安法(Cyclic voltammetry,CV)、计时电流法(Chrono-amperometry,CA)和方波伏安法(Squarewa vevoltammetry,SWV)。结果:INH在碳糊电极(Carbon paste electrode,CPE)上的直接电化学氧化过程十分迟缓,MPT/CPE对INH电化学氧化具有良好的催化作用。同时测定了INH在MPT/CPE上的电极过程动力学参数,用方波伏安法(SWV)测得催化氧化峰电流与INH在5.0×10-7～1.0×10-4mol.L-1浓度范围内呈良好线性关系,线性回归方程为Ipa(μA)=186.63C(10-3mol.L-1)+30.30,r=0.9971,检出限为2.1×10-7mol.L-1(S/N=3)。结论:本方法灵敏度高,操作简单,可用于INH电化学定量测定。
Objective：Electrochemical behaviors,electrochemical kinetic parameters and electroanalysis of isoniazid （INH） at carbon paste electrode（CPE） and 10-methylphenothiazine modified carbon paste electrode （MPT/CPE） were investigated.Methods：Cyclic voltammetry（CV）,chronoamperometry（CA） and square wave voltammetry（SWV）.Results：It was found that isoniazid itself showed a poor electrochemical response at carbon paste electrode （CPE）,the electrochemical behaviors could be greatly improved by using MPT/CPE,which enables a sensitive electrochemical determination of the substrate isoniazid.The kinetic parameters of the reaction were evaluated.The catalytic oxidation peak current of isoniazid versus its concentration had a good linear relationship in the concentration range of 5.0×10-7-1.0×10-4 mol/L-1with the correlation coefficient of 0.9971,and the detection limit of 2.1×10-7 mol/L-1 by Square wave voltammetry（SWV）.Conclusion：The result can be applied in isoniazid electrochemical determination with both low detection limit and high sensitivity.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第2期325-328,共4页
Chinese Journal of Pharmaceutical Analysis
基金
宁夏高等学校科学研究项目(200623)资助
关键词
异烟肼
10-甲基吩噻嗪
碳糊修饰电极
电催化氧化
电分析方法
Isoniazid
10 - methylphenothiazine
modified carbon paste electrode
electrocatalytic oxidation
electro- chemical determination
