摘要
目的建立测量兔房水中及离体角膜灌流接收池中的冰片含量的气相色谱测定方法。方法以萘为内标,乙酸乙脂萃取样本;色谱条件:OV-1701石英毛细管柱;进样口温度:210℃;柱温:130℃;FID温度:250℃;载气:氮气,3.0psi;氢气:45mL/min;空气:450mL/min;分流比:10:1。结果龙脑和异龙脑分离良好,龙脑和异龙脑在浓度0.2～20μg/mL之间与内标峰面积之比线性良好,R2分别为0.9953,0.9902;日内及日间的RSD小于9.68%和12.60%。结论该方法简便,定量准确,可用于冰片眼科制剂的研究。
Objective To establish gas chromatography for determining the contents of borneol in aqueous humor and perfusate of rabbit cornea. Methods Naphthalene was used as the internal standard, samples were extracted by ethyl acetate and the chromatographic procedure was carried out in OV - 1701 capillary column. The parameters were set as follows： Inject Temp, 210℃; Oven Temp, 30℃; FIDTemp, 250%; Carrier gas： Nitrogen, 3.0psi; Hydrogen, 45 ml/min; Air, 450ml/ min; split ratio, 10： 1. Results Borneol and isoborneol were well separated from other components. Borneol and isoborneol had an good linearity in the rang of 0.2 - 20 μg/ml, R^2 was 0. 9953 for borneol and 0. 9902 for isoborneol; and the intra - day and inter- day RSD were less than 9.68 % and 12.60 % respectively. Conclusion The method is simple and precise, and can be used for determining borneol in ophthalmological preparation.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第6期545-547,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省高等学校中药有效性与安全性重点实验室开放基金(kf08002)
关键词
冰片
气相色谱法
房水
含量测定
Borneol
Gas chromatography
Aqueous humor
Quantitative analysis
