摘要
目的：建立GC法测定测定消曚眼膏中龙脑和异龙脑的含量。方法：采用DB-WAX色谱柱（柱长30m,内径0.53mm,膜厚度1μm）,载气为氮气,分流比为10：1,柱温为140℃,进样口温度为180℃,检测器温度为200℃。结果：龙脑在0.0317~0.5076μg范围内线性关系良好（r=1.0000）,平均加样回收率为99.22%（n=9）,RSD为1.69%;异龙脑在0.0196~0.3136μg范围内线性关系良好（r=0.9991）,平均加样回收率为98.32%（n=9）,RSD为2.22%。结论：本法灵敏、准确,可作为消曚眼膏的质量控制指标。
Objective： To establish GC method for determination of borneol and isoborneol in Xiaomeng eye ointment. Methods： The DB -WAX chromatographic column （30m × 0. 53mm ×11μm） was adopted with nitrogen as the carrier gas and the split ratio of 10： 1. The column temperature was 140℃, the injection port temperature was 180℃ and the detector temperature was 200℃. Re- suits： The linear range of borneol was in the range of 0. 0317 ~ 0. 5076μg （r = 1. 0000）, and the average recovery （n = 9 ） was 99. 22% and RSD was 1.69% ; The linear range of isoborneol was in the range of 0. 0196 - 0. 3136ttg （ r = 1. 0000）, and the average recovery （n = 9） was 98.32% and RSD was 2. 22%. Conclusion： This method is convenient, rapid, accurate and reproducible, and can be used for quality control of Xiaomeng eye ointment.
出处
《中国民族民间医药》
2015年第20期14-15,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
广西"八桂学者"工程专项经费资助
广西自然科学基金资助项目(2011GXNSFF018006)
关键词
GC
消曚眼膏
龙脑
异龙脑
GC
Xiaomeng eye ointment
Borneol and Isobomeol
