摘要
目的用气相色谱法同时检测眼用制剂中各种清凉剂含量。方法色谱条件:OV-1701石英毛细管柱;进样口温度:220℃;炉温:105℃;FID检测器温度:250℃;载气:氮气,6.0 psi;氢气:45 mL/min;空气:450 mL/min;分流比:10∶1;以乙酸乙酯萃取样本,正十三烷为内标。结果樟脑、异龙脑、薄荷醇、龙脑分离良好,在浓度2.0～250.0μg.mL-1间与内标峰面积之比线性良好,方法学考察结果符合要求。结论该方法定量准确,可用于眼用制剂中清凉剂辅料的研究。
Objective To establish a gas chromatography（GC） method for simultaneous determination of algefacients in ophthalmic preparation. Methods The chromatographic procedure was carried out in OV-1701 capillary column. The chromatographic conditions were as follows： injection temperature at 220 ℃, oven temperature at 105 ℃, FID detector temperature at 250 ℃, carrier gas being nitrogen 6.0 psi, hydrogen flow rate at 45mL/min, air flow rate at 450 mL/min, and split ratio being 10 ： 1. Samples were extracted by ethyl acetate, and tridecane was used as the internal standard substance. Results Camphor, isoborneol, menthol and borneol were separated from other compo nents of ophthalmic preparation, and they had good linearity in the rang of 2.0-250.0 ug·mL-1. All items of empirical method conformed to requirements. Conclusion The established method is precise and effective, and can be used for qualitation and quantitation of algefacient components in ophthalmic preparation.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2012年第2期190-193,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省"211工程"三期重点学科建设项目"药物警戒系统中的中药再评价研究"(粤发改社[2009]972号)
广东省建设中医药强省课题(2010373)
广东省自然科学基金(S2011040003546)
广东省科技计划项目(2011B031700069)
关键词
清凉剂
眼用制剂
冰片
气相色谱法
正十三烷
Algefacient
Ophthalmic Preparation
Borneol
Gas Chromatography
Tridecane
