摘要
应用嗜鼠心肌ＣｏｘｓａｃｋｉｅＢ３病毒（ＣＶＢ３ｍ）给ＢＡＬＢ／Ｃ小鼠重复腹腔注射，连续３次，间隔３０天，末次注射后１０天处死小鼠。光学显微镜检查发现，小鼠心肌可见不同时期的病灶（坏死灶、瘢痕），心肌损伤检出率达１００％。应用ＴＵＮＥＬ法检测发现，８２．５０％感染组鼠心肌中可见散在心肌细胞凋亡，与对照组（２３．０８％）比较，Ｐ＜０．０１，有极显著性差异。提示ＣＶＢ３ｍ重复感染可致ＢＡＬＢ／Ｃ小鼠慢性心肌损伤，心肌细胞凋亡参与心肌损伤的发生与发展。
The tropism mice myocardium Coxsackie B 3 virus (CVB 3m ) was reinjected into BALB/C mice.The injection was for 3 times and there was an interval of 30 days between the two injections.The mice were executed after 10 days of the last injection.The repeated injury and repaired focal myocardial injury were found under the optical microscope.The rate of myocardial injury was 100%.The 82.50% of infectious mice group was found apoptosis of myocardial cell scattered in the mice myocardium with TUNEL method.The significant difference was found (P<0.01) compared with that of control group (23.80%).The results suggested that chronic myocardial jnjury was induced by CVB 3m reinfection and apoptosis of myocardial cell participated in occurrence and development of myocardial injury.
出处
《中国地方病学杂志》
CAS
CSCD
1998年第6期349-351,共3页
Chinese Jouranl of Endemiology
基金
黑龙江省"九五"攻关课题
