摘要
应用嗜鼠心肌Coxsackie B_3病毒(CVB_(3m))给BALB/C小鼠重复腹腔注射,连续3次,间隔30d,末次注射后10d处死小鼠。光学显微镜检查发现,小鼠心肌可见不同时期的病灶(坏死灶、瘢痕灶),心肌损伤检出率达100％。应用免疫组化法检测发现,72.50％感染组鼠心肌中可见NOS_Ⅱ蛋白的表达,与对照组(26.92％)比较,P<0.01,有极显著差异。提示CVB_(3m)重复感染可致BALB/C小鼠慢性心肌损伤,NO参与心肌损伤的发生与发展。
The tropism mice myocardium Coxsackie B3 virus(CVB3m) was reinjected for BALB/C mice. The injection was 3 times and there was an interval of days between the two injection. The mice were executed after 10 days in the last injection. The myocardial injure of differential time was found by the optical microscope. The rate was 100% . The 72.50% of infectional mice group was found expression of rate in NOS in the myocardium with immunohistochemical method. The significant difference was found( P < 0.01)comared with control group(26.92%) .The results suggested that BALB/C mice chronic myocardial injure was induced by reinfection CVB3m, and NO participate in occurrence and development of myocardial injure.
出处
《中国地方病防治》
CAS
1998年第3期137-138,共2页
Chinese Journal of Control of Endemic Diseases
基金
黑龙江省九五攻关课题
