摘要
本文用抗原特异性细胞毒技术检测小鼠感染coxsackie B_3病毒后并腹腔注射黄芪一周在5—28天脾脏、外周血及心肌中T淋巴细胞亚群分布的变化情况。结果显示:黄芪治疗组小鼠外周血、脾脏中Thy1+细胞百分率在感染后第7—14天明显增高(P<0.05),在心肌中则低于同期生理盐水组。L3T4+淋巴细胞百分率黄芪治疗组在病毒感染后5—14天的脾脏,第9天的外周血和第7、14天的心肌中显著低于生理盐水组,而Lyt2+淋巴细胞百分率两实验组间均无统计学差异,提示中药黄芪对急性病毒性心肌炎小鼠T细胞免疫具有明显的调节作用,且主要是通过调节Thy1+和L3T4+淋巴细胞亚群而起到治疗目的的。
Effect of Astragalus Membranaceus(AM) in lymphocyte subsets in an animal model of viral myocarditis were investigated by antigen specific cytotoxicity assay, The percentage of Thyl+cell in the peripheral blood and spleen was significantly higher in AM treated mice than in normal saline(NS) treated mice on 7th, 9th, 14th day(P<0.05) after Coxsackie B3 viral(CB 3V) inoculation, while it was statistically lower in myocardium in AM treated mice on 14th day after infection The percentage of L3T4+cell was markedly decreased in AM treated mouse spleen cells on 5—14th day postCB_3V inoculation, while it was significantlly lower in AM treated mouse peripheral blood and myocardium on 9th day, and 7th, 14th day, respectively. In contrast, the percentage of Lyt2+cell was no statistical significance between two groups through the whole experiments. The results suggest that traditional Chinese medical-AM can regulate T lymphocytes, especially Thyl + and L3T4+cells, mediated immune reaction in acute viral myoearditis.
出处
《中国病毒学》
CSCD
1992年第2期129-133,共5页
Virologica Sinica
基金
国家自然科学基金
关键词
病毒性心肌炎
T细胞
Coxsaekie B3 virus Acute viral myoearditis Astragalus Membranaceus Thyl+lymphocyte(pan-T) Lyt2+lymphocyte(Tc/s) L3T4+lymphocyte(Th/i)
