摘要
本文研究了基因型、植物生长调节剂及配比、基本培养基、外植体和苗龄等因子对草莓离体再生不定芽的影响。结果表明：草莓不同基因型再生能力差异很大，再生频率为0-94％，在4个供试草莓品种中以“达斯莱克特”再生能力最强。基本培养基MS＋B5大大促进草莓“达斯莱克特”叶片不定芽的分化，以MS＋B5＋6-BA3．0mg／L＋2，4-D0．1mg／L诱导效果最佳；同时，低浓度的2,4-D有利于不定芽再生，IAA、IBA、NAA分别以浓度1．0mg／L、0．5mg／L、0．1mg／L诱导较好，TDZ的诱导效果不明显，且不及6-BA。另外，叶片再生能力远大于叶柄和根，根未诱导出不定芽，以继代28～35d的试管苗幼嫩、平展叶片作为接种外植体为宜，获得了草莓高效的离体再生体系。
In this research, we have studied the effects of a series of cultural factors （genotypes, hormone concentrations, basic media, leaf ages and explants） in the inducing adventitious shoot of strawberry, respectively. The strawberry regeneration system has been established successfully. The results revealed that regeneration frequency varied dramatically with genotype of strawberry, ranging from 0 to 94%. Of four strawberry cultivars, the regeneration frequency of Dasrlect was the most highest, whereas the strawberry Liyinv was observed to be very difficult to regenerate. The combination of 6-BA 3.0 mg/L and 2,4-D 0.1 mg/L were found to be the most efficient of Dasrlect. And 2,4-D was better than IAA, IBA and NAA, all of2,4-D, IBA and NAA with high concentration were not beneficial to induce shoot of Dasrlect. In addition, it had no obvious effects on the shoot formation of Dasrlect with TDZ at the levels tested in this study. And the regeneration frequency was significantly enhanced by the MS＋B5 basic medium. The regeneration capacity of leaf was higher far than petiole and root. Leafage can affect also the regeneration frequency and 28-35 d was the best. The maximum regeneration frequency of petiole only got 40%, whereas the roots did not induce any advenvious bud.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2009年第4期770-774,共5页
Genomics and Applied Biology
基金
宿州学院硕士启动基金项目(2007yss13)资助
关键词
草莓
离体再生
IAA
IBA
NAA
Strawberry （Fragaria ananassa Duch.）, Regeneration in vitro, IAA, IBA, NAA
