摘要
研究影响农杆菌介导的苹果主栽品种遗传转化的若干因子效应，建立起新乔纳金苹果简单、高效的遗传转化系统。外源基因向新乔纳金苹果细胞转移的效率明显高于皇家嗄拉和富士苹果。菌株ＥＨＡ１０５介导基因转移的能力比菌株ＬＢＡ４４０４明显高。用乙酰丁香酮、磷酸饥饿、低ｐＨ值等ｖｉｒ基因诱导因子处理菌株ＥＨＡ１０５的细胞，未能提高转化效率。叶片在菌液中的浸泡时间不仅影响基因转移效率，而且影响头孢霉素对外植体上农杆菌生长的抑制效果。共培养时，叶片远轴面与培养基接触比近轴面与培养基接触的转化效率高。
? Several factors that affect genetic transformation of the commercial apple cultivars were examined. A simple and efficient genetic transformation system was developed. The efficiency of exogenous genes transferring into cells of apple cultivar New Jonagold was obviously higher than the efficiency of exogenous genes transferring into cells of apple cultivar Royal Gala or Fuji. The ability of strain EHA 105mediated gene transfer was higher remarkably than the ability of strain LBA 4404. Inducing vir gene expression factors, such as acetosyringone, phosphate starvation and low pH, had no obvious effect on the efficiency of strain EHA105mediated transformation. The time of dipping leaf pieces in suspension affected not only the gene transfer efficiency,but also the inhibition by cefotaxime of the growth of Agrobacterium on explants. The transformation efficiency of leaf pieces which abaxial side contacted with medium during concultivation was higher than the efficiency of leaf pieces which adaxial side contacted with medium.
关键词
苹果
根癌农杆菌
基因表达
高效遗传转化
Malus×domestica, Transformation, Agrobacterium tumefaciens, Gene expression
