摘要
以适合东北地区生长的“丹莓1号”草莓优良品种为研究对象,系统开展脱毒组培快繁技术研究。以“丹莓1号”草莓的茎尖为外植体,考察了不同植物激素对于不定芽诱导、增殖及生根的影响,同时也考察了移栽基质对于组培苗驯化成活的影响,确定了MS+1 mg·L-16-BA+0.1 mg·L-12,4-D为茎尖不定芽诱导的最佳培养基,MS+1 mg·L-16-BA+0.3 mg·L-1 NAA为不定芽增殖的最佳培养基,MS+0.4 mg·L-1 IBA为不定芽生根的最佳培养基,苗圃土∶[KG-*2/3]蛭石∶[KG-*2/3]珍珠岩=1∶[KG-*2/3]2∶[KG-*2/3]1为草莓组培苗成活率最高的移栽基质。本研究为“丹莓1号”优良草莓品种脱毒苗的规模化繁育奠定了理论基础。
The superior“Dan Mei 1”strawberry variety that is suitable for growing in Northeast China were selected as the research object,and the tissue culture technology for detoxification and rapid propagation of strawberry was carried out systematically.The effects of different plant hormones on adventitious bud induction,proliferation and rootage were investigated using the stem tip of“Dan Mei 1”strawberry as explants.Also,the effects of transplanting medium on the domestication survival of strawberry plantlets were investigated.The results showed that MS with 1 mg·L-16-BA+0.1 mg·L-12,4-D was suitable for adventitious bud induction.The best medium for adventitious bud proliferation was[JP3]MS with 1 mg·L-16-BA+0.3 mg·L-1 NAA.And,MS with 0.4 mg·L-1 IBA was the best medium for[JP]adventitious bud rootage.Perlite∶[KG-*2/3]vermiculite∶[KG-*2/3]nursery soil with the ratio of 1∶[KG-*2/3]2∶[KG-*2/3]1 was the best transplanting medium for the domestication of strawberry plantlets.Overall,this study provided a theoretical foundation for the large-scale propagation of the virus-free“Dan Mei 1”strawberry variety.
作者
王馨
盖庆岩
焦骄
付玉杰
刘婧
王紫莹
WANG Xin;GAI Qing-Yan;JIAO Jiao;FU Yu-Jie;LIU Jing;WANG Zi-Ying(Key Laboratory of Forest Plant Ecology,Northeast Forestry University,Harbin 150040)
出处
《植物研究》
CAS
CSCD
北大核心
2020年第1期153-160,共8页
Bulletin of Botanical Research
基金
中央高校基本科研业务费专项资金项目(2572017DA04,2572018BU02)~~
关键词
“丹莓1号”草莓
茎尖分化
快速增殖
脱毒苗
“Dan Mei 1”strawberry
stem tip differentiation
rapid proliferation
virus-free plantlets
